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Immunochromatographic lateral flow to detect natamycin

Immunochromatographic lateral flow is a method that has been specially developed to detect natamycin in samples of milk and cheese. To do so, a monoclonal antibody combines with nanoparticles of gold, which acts as the main reagents to determine whether natamycin is present.

A capture reagent is also established by using carbodiimide and N-hydroxysuccinimide, which are immobilised on a nitrocellulose membrane.

This semi-quantitative method only requires a test time of 15 minutes for completion. Thanks to this method, it is possible to observe the optimum concentrations of antigens in coatings for milk and yoghurt samples, the optical density, and the visual limit for detecting natamycin in them.