Readers ask: Why Is It Critical To Use Aseptic Technique When Transferring The Antibiotic Disks To The Agar?


Why is it important to keep the antibiotic disc fully contact with agar surface?

As a consequence, the prongs that press the discs into place on the agar surface can become contaminated which can then contaminate successive plates. If insufficient contact between the discs and the agar takes place moisture may not be drawn into the disc and the antibiotic would not diffuse into the plate.

Why is it important to measure the size of the zone of inhibition when determining antibiotic sensitivity for an organism quizlet?

The larger the clear zones, the more effective the antibiotic is. DETERMINE THE BACTERIAL POPULATIONS SENSITIVITY TO A RANGE OF ANTIBIOTICS. This can be done by placing small disks of filter paper or agar impregnated with various types of antibiotics onto the bacterial lawn.

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What is the purpose of the antibiotics in the agar plate quizlet?

The purpose of the LB agar with to antibiotics was to make sure that no bacteria grew at all.

How does the antibiotic get from the disk into the agar?

How does the antibiotic get from the disk into the agar? The antibiotic diffuses out of the disk and into the agar. This diffusion can be affected by temperature and depth of agar in the plate. After incubation, does the antibiotic extend into the agar beyond the zone of inhibition?

What does the Zone of Inhibition tell you?

The Zone of inhibition is a circular area around the spot of the antibiotic in which the bacteria colonies do not grow. The zone of inhibition can be used to measure the susceptibility of the bacteria to wards the antibiotic.

What are the factors that affect the zone of inhibition?

Factors that affect zones of inhibition:

  • Pathogen susceptibility. Selection of the antibiotic is based on the type of organism being tested.
  • Antibiotic diffusion effects. The rate of diffusion of an antibiotic through the agar is not always same.
  • Agar depth.
  • pH.
  • Size of the inoculated organism:
  • Presence of other metals:

Are antimicrobials useful in viral infections?

Antibiotics do not work on viruses, such as those that cause colds, flu, bronchitis, or runny noses, even if the mucus is thick, yellow, or green. Antibiotics are only needed for treating certain infections caused by bacteria, but even some bacterial infections get better without antibiotics.

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What does a large zone of inhibition mean?

Large zones of inhibition indicate that the organism is susceptible, while small or no zone of inhibition indicate resistance. An interpretation of intermediate is given for zones which fall between the accepted cutoffs for the other interpretations.

What are the factors considered in the standardization of the antibiotics sensitivity test?

The main factors thought to affect reproducibility of susceptibility testing include inoculum, media composition and depth, delay between application of the disc and incubation, temperature, atmosphere and duration of incubation, generation time, the antibiotic concentration of the disc and the method of reading zone

What is the purpose of the antibiotics in the agar plate?

The addition of an antibiotic to this gel allows for the selection of only those bacteria with resistance to that antibiotic – usually conferred by a plasmid carrying the antibiotic resistance gene. The following protocol will allow you to make your own LB/ agar plates with your antibiotic of interest.

What is the purpose of a sterile spreader?

1 Sterile spreaders are used to distribute inoculum over the surface of prepared agar plates. You can sterilise a wrapped glass spreader in a hot air oven or sterilise by flaming with alcohol.

Which antibiotics were not effective at all against E coli quizlet?

The streptomycin antibiotic is not effective on E. coli I as it is resistant to it, so it continued growing on the agar plate. It was not resistant to ampicillin, so the ampicillin destroyed the bacteria.

Which patients infection appears to be the most difficult to treat Why?

Patient B’s infection appears to be the most difficult to treat because it was resistant to 3 differentantibiotics and only sensitive to Streptomycin and Gentamicin.

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What would happen if we didn’t use aseptic technique to put the antibiotic disk on the plate?

What would happen if we didn’t use aseptic technique to put the antibiotic disk on the plate? In these cases, changes in antibiotic concentration might not show as much effect as one would expect. Antibiotics can be bactericidal ( they kill the microbe) or bacteriostatic ( they inhibit microbial growth, but do not kill).

Which antibiotic was most effective in inhibiting the growth of E coli?

Tetracycline was the most effective true antibiotic tested against E. coli.

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